Mitochondrial pellet was resuspended in digitonin buffer (30 mM HEPES-KOH, pH 7.4, 150 mM potassium acetate, 10 [v/v] glycerol, and 1 mg of digitonin) and kept on ice for 30 min. Ten microliters of antibody serum, 50 mL of Protein A-Sepharose, 1 (w/v) BSA, and Complete protease inhibitor (Roche) were added to the mitochondria and incubated for 6 h at 4 on a rotating wheel at 20 rpm. Beads were

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S that have been suggested include delayed or prolonged neutropenia [46], and pneumonia [48]. G-CSF should be avoided in this context (cf infra).Zafrani and Azoulay BMC Infectious Diseases 2014, 14:512 http://www.biomedcentral.com/1471-2334/14/Page 5 ofCatheter removalDeciding when to remove CVC is a common problem in neutropenic patients in ICU. In patients with bacteremia due to Enterobacteriace