Mitochondrial pellet was resuspended in digitonin buffer (30 mM HEPES-KOH, pH 7.4, 150 mM potassium acetate, 10 [v/v] glycerol, and 1 mg of digitonin) and kept on ice for 30 min. Ten microliters of antibody serum, 50 mL of Protein A-Sepharose, 1 (w/v) BSA, and Complete protease inhibitor (Roche) were added to the mitochondria and incubated for 6 h at 4 on a rotating wheel at 20 rpm. Beads were

Ly increasing. This audit aimed to look retrospectively at our admissions to Intensive Care, to categorise them into frail or non frail, and evaluate how frailty correlated with ICU length of stay and mortality Methods: A retrospective case note review of all patients admitted to Intensive Care over a six month period in the Victoria Infirmary and then Queen Elizabeth University hospital in Glasgo

Ly increasing. This audit aimed to look retrospectively at our admissions to Intensive Care, to categorise them into frail or non frail, and evaluate how frailty correlated with ICU length of stay and mortality Methods: A retrospective case note review of all patients admitted to Intensive Care over a six month period in the Victoria Infirmary and then Queen Elizabeth University hospital in Glasgo

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Ies show that frailty is associated with increased mortality so it is indeed interesting that this audit has shown no difference between the two groups.References 1. Rockwood, Song, McKnight. A global clinical measure of fitness and frailty in elderly people.CMAJ: 2005, vol 173 no.5 2. The Edmonton Frailty Scale. Age and Ageing, volume 35.A940 Outcomes in elderly patients admitted to ICU C. Castro